Catalog Number: RPT0201 

CAS: 9002-07-7
EC: 3.4.21.4 

Storage temperature: 2-8℃ 

Source: Recombinant trypsin is a genetically engineered protein expressed in E.coli  

Protein Sequence:The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin 

1.DESCRIPTION 

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA. 

2.MAIN FEATURES

UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A₂₅₃ of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path). 

3.RECOMMEND USAGE

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.  

4.STABILITY OF STORAGE AND TRANSPORT 

Stability of storage: Recombinant trypsin lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months. It should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing. 

Stability of transport: The product is stable by blue ice insulation transport. 

5.PRODUCT ADVANTAGE 

Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process. 

Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable. 

High purity: Higher specific activity.Host protein residues is less than the limits of biological products. 

Lyophilized powder: The product is lyophilized powder and is easy to store and transport. 

Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

Complete quality documents: we can provide relevant regular support files in according to customers’ requirement. 

6.PRODUCT USAGE 

The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes.

7.RELATED PRODUCTS 

Recombinant carboxypeptidase B 

Sequencing grade trypsin 

Recombinant human trypsin 

Recombinant enterokinase